水生动物热休克蛋白研究进展   总被引：8，自引：2，他引：6  

董云伟  董双林  纪婷婷 《中国海洋大学学报(自然科学版)》2008,38(1):39-44

由于栖息环境的特殊性,水生动物热休克蛋白的诱导及其生态意义具有不同于陆生动物的特性.本文总结了近年来水生动物热休克蛋白方面的研究进展,介绍热休克蛋白的调节机制及其功能,归纳水生动物热休克蛋白对温度、环境污染和其他因子的反应,着重分析热休克蛋白表达在水生动物中热耐受性和交叉保护中的作用,及其对水生动物分布和适合度的影响.  相似文献   

Study on Application of Polyenzyme Method to Offal of Harengula zunasi     

邓尚贵  杨萍  夏杏洲 《中国海洋湖沼学报》2003,21(4):312-318

The new polyenzyme method for making gravy from Harengula zunasi offal involves protein enzymolysis with flavorase after proper alkaline and neutral protease levels were established by orthogonal trials to select the best hydrolytic conditions for processing offal with alkaline and neutral protease. The conditions for the polyenzyme method were pH of 7.0, temperature of 50℃ , alkaline and neutral protease concentration of 1.5% respectively, hydrolysis time of 120 min, andflavorase concentration of 2.0% , for 60 min. The new gravy-making technology yields a nutritious and delicious gravy containing 40.3 % of total essential amino acids, with delicious amino acids Glu, Asp, Gly, Ala, Pro and Ser comprising 49.5% , total and amino nitrogen being respectively1.9 and 1.1 g/100 g (amino acid nitrogen being 61.0% of total nitrogen), The polyenzyme method was used to make 14.8% protein gravy from Harengula zunasi offal. In addition, in inorganic elements, the phosphorus content is the highest.  相似文献   

Study on application of polyenzyme method to offal of Harengula zunasi     

Deng Shanggui  Yang Ping  Xia Xingzhou 《中国海洋湖沼学报》2003,21(4):312-318

The new polyenzyme method for making gravy fromHarengula zunasi offal involves protein enzymolysis with flavorase after proper alkaline and neutral protease levels were established by orthogonal trials to select the best hydrolytic conditions for processing offal with alkaline and neutral protease. The conditions for the polyenzyme method were pH of 7.0, temperature of 50°C, alkaline and neutral protease concentration of 1.5% respectively, hydrolysis time of 120 min, and flavorase concentration of 2.0%, for 60 min. The new gravy-making technology yields a nutritious and delicious gravy containing 40.3% of total essential amino acids, with delicious amino acids Glu, Asp, Gly, Ala, Pro and Ser comprising 49.5%, total and amino nitrogen being respectively 1.9 and 1.1 g/100 g (amino acid nitrogen being 61.0% of total nitrogen), The polyenzyme method was used to make 14.8% protein gravy fromHarengula zunasi offal. In addition, inorganic elements, the phosphorus content is the highest. This project was aided financially by the Guangdong Science and Technology Office (No. 2KM06002S).  相似文献   

Purification and characterization of an alkaline protease from Acetes chinensis     

Xu Jiachao  Liu Xin  Li Zhaojie  Xu Jie  Xue Changhu  Gao Xin 《中国海洋大学学报(英文版)》2005,4(3):257-261

An alkaline protease from Acetes chinensis was purified and characterized in this study. The steps of purification include ammonium sulfate precipitation, ion-exchange chromatography with Q-sepharose Fast Flow, gel filtration chromatography with S300 and the second ion-exchange chromatography with Q-sepharose Fast Flow. The protease was isolated and purified, which was present and active on protein substrates (azocasein and casein). The specific protease activity was 17.15 folds and the recovery was 4.67. The molecular weight of the protease was estimated at 23.2 kD by SDS-PAGE. With azocasein as the susbstrate, the optimal temperature was 55°C and the optimal pH value was 5.5. Ion Ca²⁺ could enhance the proteolytic activity of the protease, while Cu²⁺, EDTA and PMSF could inhibit its activity.  相似文献   

双链DNA片段的碱基空间模式BSP——基因组调控区的一种可能的信息编码方式     

李许卿 《天文研究与技术》2002,(1)

生物体可看作是一种特殊的信息处理系统 ,论文的第二章从这种观点出发讨论了生物细胞内最大的信息源基因组DNA序列的信息编码问题。首先探讨了基因组蛋白质编码区的信息编码问题 ;简要回顾转录水平的基因表达调控后 ,讨论了基因组基因表达调控区的信息编码问题。第三章介绍了我们利用internet上可获取的开放的生物信息学资源 ,对基因组DNA调控区的一种可能的编码方式所进行的理论研究。这种可能的编码方式我们称之为双链DNA上的碱基空间模式BSP (BaseSpatialPattern)。首先 ,我们发现了由三联体BSP介导的一种 4与 2 0的关系 ,称为 4 -BSP -2 0关系。并推测 4 -BSP -2 0关系也许是某些DNA—蛋白质相互作用中的一种识别密码 ;第二 ,研究真核细胞转录调控中的DNA—蛋白质相互作用的实验数据表明 :某些DNA结合蛋白质对双螺旋核酸的专一性识别可能与BSP有关。并找到了 7组由其他生物学家公开发表的实验数据支持这一猜测 ;第三 ,研究了BSP的形式化描述问题 ,找到了一种基于等价类的BSP描述方法 ,并开发了相应的计算机处理算法。该描述方法简明且便于用计算机进行处理 ,更重要的是它提供了一种研究途径 ,可把BSP和 4 -BSP -2 0关系推广到单链DNA (ssDNA)和ssDNA -蛋白质相互作用的研究中 ,从而可进一步推广到RN  相似文献   

BP神经网络在局部似大地水准面精化的应用   总被引：1，自引：0，他引：1  

李爱国  WANG Wei  徐永胜 《全球定位系统》2008,33(3):29-31

针对高程拟合中的模型误差问题,采用BP神经网络方法进行拟合,可以减小模型误差,提高似大地水准面的精化精度。在焦作市局部似大地水准面精化算例中,BP神经网络高程拟合取得了良好的效果。  相似文献   

城镇黑臭河道治理:多水塘活水链人工湿地的应用     

杨棠武  熊依依  忻飞  邵一奇  戈萍燕  徐锋  张轩波  安树青 《湿地科学与管理》2021,(1):51-55

随着现代工业的发展,城市黑臭河道问题日趋严重,严重影响了城市形象、生态环境和居民身心健康。利用多水塘活水链人工湿地技术净化江苏常州永胜河河水水质。监测结果显示:该技术使水体总氮浓度削减了92.0%、总磷浓度削减了82.9%,处理后的水体总氮、总磷含量达到地表IV类水标准。项目年处理水量约30万t,每年以较低的成本(平均水处理费用约为0.03元/t),削减总氮、总磷和氨氮量分别为1496.6 kg、176.0 kg和1408.6 kg。通过引植多种浮叶植物、挺水植物、沉水植物,该湿地修复工程为当地营造了优美的湿地景观。多水塘活水链人工湿地是集经济效益、生态效益、环境效益于一体的新型水体生态治理技术,为我国城镇黑臭河道治理提供了一条新的途径。  相似文献   

Effect of Bacillus mucilaginosus on weathering of phosphorite and a preliminary analysis of bacterial proteins   总被引：3，自引：0，他引：3  

Shu Chen  Bin Lian  Congqiang Liu 《中国地球化学学报》2008,27(2):209-216

The authors investigated the effect of Bacillus mucilaginosus on weathering of phosphorite. Analysis of different proteins was of significance in exploring the molecular biological mechanism in the bacterial weathering process. The concrete methods are described as follows： Mineral powder was put into liquid culture medium and B. mucilaginosus was incubated in the medium. The control （group） had no mineral powder in the medium. The treatments and controls were cultured simultaneously under the same condition. In a few days, the supernatant was filtrated, the main cations （Ca^2＋, Mg^2＋, Na^＋, Mn^2＋, Al^3＋, Fe^3＋, K^＋） were measured by ICP-OES, and the contents of water soluble phosphorus （Pws） and silicon （Siws） were determined by colorimetry. The residual solid was weighed on the filter paper, followed by digestion with concentrated HNO3. The concentrations of the main cations and Pws, Siws in the digest liquid were measured by using the method mentioned above. After the supernatant was centrifuged, the precipitation was used to analyze the protein differences between the treatment groups and the control groups by 2-dimentional gel electrophoresis （2-DE）. The experimental results showed that apatite and quartz were partially weathered, but kaolinite was dissolved completely. The population of bacteria increased when mineral powder was added in the liquid medium. Software analysis and comparison of the 2-DE pictures of bacterial proteins revealed 1134 visible protein spots in the treatment group, and 729 visible protein spots in the control group. To compare the bacterial protein expression contents of the treatment group with those of the control group, there were 496 different protein spots, including 214 protein spots which indicated that the protein contents increased, 75 protein spots were indicative of a decrease, and 207 proteins were newly synthesized. It is proposed that the increased bacterial contents may be related to some protein expression and activation after the  相似文献   

小球藻紫外线诱变及高产藻株筛选   总被引：1，自引：0，他引：1  

张学成  时艳侠  孟振 《中国海洋大学学报(自然科学版)》2007,37(5):749-753

利用单细胞分离技术和紫外诱变育种技术,筛选高产小球藻株。通过单细胞分离技术获得的藻株Chlorella vul-garisQ7,生长速度和蛋白含量均优于其它藻株。以C.vulgarisQ7为出发株,进行紫外诱变,从120株诱变单克隆中筛选出3个株系:M51,M59,M73。与出发株相比,突变株M51,M59,M73的生长速率分别提高了6.23%,3.8%,5.92%。蛋白含量分别提高了2.5%,3.1%,1.9%。研究了诱变株的最适生长条件,结果表明在140μmol.m-2.s-1,25℃时,诱变株具有最大的生长速率和生物量。探讨了诱变株的遗传稳定性,结果表明诱变株可稳定遗传。  相似文献   

棉粕蛋白酶解物对异育银鲫(Carassius auratus gibelio)消化、生长和胰蛋白酶mRNA表达量的影响     

刘文斌  王恬 《海洋与湖沼》2006,37(6):568-574

以棉粕蛋白为酶解底物,用枯草杆菌蛋白酶对其进行酶解,以酶解产物1.5%和3.0%两个梯度等量替代鱼饲料配方中棉粕,在室内流水养殖系统中喂养异育银鲫鱼种[体重为(30±2)g]65天。测定鱼的生长、营养物质表观消化率、消化蛋白酶活性及肝胰脏中胰蛋白酶mRNA表达水平等指标。结果表明,添加1.5%和3.0%棉粕酶解产物的鱼在饲养35天后的特定增长率(SGR)分别比对照组高32.5%和56.7%,且差异显著(P<0.05);在饲养65天后,两组特定增长率分别比对照组高8.0%和21.0%,且差异极显著(P<0.01),肝胰脏中胰蛋白酶mRNA表达水平也随棉粕酶解产物添加梯度提高而相应提高,表明鱼的生长与消化蛋白酶mRNA表达水平相关。同时棉粕蛋白酶解物对肠道蛋白酶的活性和营养物质表观消化率都有促进作用,而棉粕蛋白酶解物对鱼肌肉成分并没有改变,这也表明棉粕蛋白酶解物在促进鱼生长、内源酶活性同时并未降低鱼的品质。  相似文献